Highly active enzymes by automated combinatorial backbone assembly and sequence design
oleh: Gideon Lapidoth, Olga Khersonsky, Rosalie Lipsh, Orly Dym, Shira Albeck, Shelly Rogotner, Sarel J. Fleishman
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-07-01 |
Deskripsi
Computationally designed enzymes often show lower activity or stability than their natural counterparts. Here, the authors present an evolution-inspired method for automated enzyme design, creating stable enzymes with accurate active site architectures and wild-type-like activities.