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<i>Brassica rapa</i>, a major oilseed crop in high-altitude areas, is well known for its indeterminate inflorescences. However, this experiment revealed an intriguing anomaly within the plot: a variant displaying a determinate growth habit (520). Determinate inflorescences have been recognized for their role in the genetic enhancement of crops. In this study, a genetic analysis in a determinate genotype (520) and an indeterminate genotype (515) revealed that two independently inherited recessive genes (<i>Brdt1</i> and <i>Brdt2</i>) are responsible for the determinate trait. BSA-seq and SSR markers were employed to successfully locate the <i>Brdt1</i> gene, which is localized within an approximate region 72.7 kb between 15,712.9 kb and 15,785.6 kb on A10. A BLAST analysis of these candidate intervals revealed that <i>Bra009508</i> (<i>BraA10.TFL1</i>) shares homology with the <i>A. thaliana TFL1</i> gene. Then, <i>BraA10.TFL1</i> (gene from the indeterminate phenotype) and <i>BraA10.tfl1</i> (gene from the determinate phenotype) were cloned and sequenced, and the results indicated that the open reading frame of the alleles comprises 537 bp. Using qRT-PCR, it was determined that <i>BraA10.TFL1</i> expression levels in shoot apexes were significantly higher in NIL-520 compared to 520. To verify the function of <i>BraA10.TFL1</i>, the gene was introduced into the determinate <i>A. thaliana tfl1</i> mutant, resulting in the restoration of indeterminate traits. These findings demonstrate that <i>BraA10.tfl1</i> is a gene that controls the determinate inflorescence trait. Overall, the results of this study provide a theoretical foundation for the further investigation of determinate inflorescence.