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Larval therapy with <em>Lucilia sericata</em> is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. <em>Netrin-1</em>, one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The <em>UNC-5 receptor</em> combines with a <em>netrin-1</em> signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the <em>UNC-5 receptor</em> mRNA in <em>L. sericata</em> using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the <em>UNC-5 receptor</em> was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the <em>Lucilia cuprina</em> netrin-1 <em>UNC-5 receptor</em>. The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in <em>UNC-5</em>. It lied at the interval of 26–131 aa. We identified the CDS of <em>netrin-1 UNC-5 receptor</em> in <em>L. sericata</em>. It could be applied to research activities implementing a new essential component design in wound healing.