An optimized measles virus glycoprotein-pseudotyped lentiviral vector production system to promote efficient transduction of human primary B cells
oleh: Eirini Vamva, Stosh Ozog, Els Verhoeyen, Richard G. James, David J. Rawlings, Bruce E. Torbett
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2022-03-01 |
Deskripsi
Summary: Measles virus envelope pseudotyped LV (MV-LV) can achieve high B cell transduction rates (up to 50%), but suffers from low titers. To overcome current limitations, we developed an optimized MV-LV production protocol that achieved consistent B cell transduction efficiency up to 75%. We detail this protocol along with analytical assays to assess the results of MV-LV mediated B cell transduction, including flow cytometry for B cell phenotypic characterization and measurement of transduction efficiency, and ddPCR for VCN analysis.