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Bacteria of the genus <i>Pseudomonas</i> are pathogens in both humans and animals. The most prevalent nosocomial pathogen is <i>P. aeruginosa</i>, particularly strains with elevated antibiotic resistance. In this study, a total of eighteen previously identified <i>Pseudomonas</i> species strains, were isolated from chicken. These strains were screened for biofilm formation and antibiotic resistance. In addition, we evaluated clove oil’s effectiveness against <i>Pseudomonas</i> isolates as an antibiofilm agent. The results showed that <i>Pseudomonas</i> species isolates were resistant to most antibiotics tested, particularly those from the β-lactamase family. A significant correlation (<i>p</i> < 0.05) between the development of multidrug-resistant isolates and biofilms is too informal. After amplifying the <i>AmpC</i>-plasmid-mediated genes (<i>bla_CMY</i>, <i>bla_MIR</i>, <i>DHA</i>, and <i>FOX</i>) and biofilm-related genes (<i>psld</i>, <i>rhlA</i>, and <i>pelA</i>) in most of our isolates, PCR confirmed this relationship. Clove oil has a potent antibiofilm effect against <i>Pseudomonas</i> isolates, and may provide a treatment for bacteria that form biofilms and are resistant to antimicrobials.