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Zika virus (ZIKV) is an RNA flavivirus (<i>Flaviviridae</i> family) endemic in tropical and subtropical regions that is transmitted to humans by <i>Aedes</i> (<i>Stegomyia</i>) species mosquitoes. The two main urban vectors of ZIKV are <i>Aedes aegypti</i> and <i>Aedes albopictus,</i> which can be found throughout Brazil. This study investigated ZIKV infection in mosquito species sampled from urban forest fragments in Manaus (Brazilian Amazon). A total of 905 non-engorged female <i>Ae. aegypti</i> (22 specimens) and <i>Ae. albopictus</i> (883 specimens) were collected using BG-Sentinel traps, entomological hand nets, and Prokopack aspirators during the rainy and dry seasons between 2018 and 2021. All pools were macerated and used to inoculate C6/36 culture cells. Overall, 3/20 (15%) <i>Ae. aegypti</i> and 5/241 (2%) <i>Ae. albopictus</i> pools screened using RT-qPCR were positive for ZIKV. No supernatants from <i>Ae. aegypti</i> were positive for ZIKV (0%), and 15 out of 241 (6.2%) <i>Ae. albopictus</i> pools were positive. In this study, we provide the first-ever evidence of <i>Ae. albopictus</i> naturally infected with ZIKV in the Amazon region.