Mixed pyruvate labeling enables backbone resonance assignment of large proteins using a single experiment
oleh: Scott A. Robson, Koh Takeuchi, Andras Boeszoermenyi, Paul W. Coote, Abhinav Dubey, Sven Hyberts, Gerhard Wagner, Haribabu Arthanari
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-01-01 |
Deskripsi
Structure determination of large proteins by solution state NMR is challenging due to spectral overlap. Here the authors present a labeling strategy using 2-13C and 3-13C pyruvate as carbon source for E. coli, which increases the effective resolution of triple-resonance HNCA experiments and helps to overcome this problem.