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In this work new floristic records for 12 taxa are reported for Natural Regional Reserve Sentina. Particularly 3 are new to Marche region (Cortaderia selloana, Eclipta prostrata, and Potamogeton berchtoldii). The survey was carried out from 2014 to 2015 through field research and collections. Some recorded species are particularly interesting from phytogeographical or conservational point of views as they are very rare in central Italy along the Adriatic coasts.