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The efficiency of different isolated of ectomycorrhizal fungi was estimated to evaluated the pinus seedlings production, in sandy soil. The treatment were: not inoculated, inoculated with isolated fungi Rh 117; Pt Silv.1; FSE RS (native) and F1- RS (native). The work was developed in greenhouse, in sandy soil collected in São Francisco de Assis RS. It was determined the fresh mass of aerial part and root, dry mass of aerial part, seedlings height, length and root specific superficial area, micorrhizae colonisation and level of N, P and K. The fungi F1 RS and Pt Silv.1 provided beneficial grown in the pinus seedlings in relation to fresh root especific superficial area. The micorrhizae association in this work did not improve nutrient uptake of N, P and K and the pinus seedlings aerial part development.