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Fluoride is abundant in the environment and is an essential trace element in living organisms. However, prolonged excessive fluoride intake can lead to fluorosis, which poses a threat to the reproductive health of animals and humans. Although previous research has mainly focused on animal models, the impact of fluoride on ovine follicular granulosa cells (GCs) has not been comprehensively elucidated. This study employed RNA-Seq technology to elucidate the toxic effects of fluoride on ovine follicular GCs and its mechanism of action. Culturing primary ovine follicular GCs in vitro and subjecting them to fluoride treatment revealed 3218 differentially expressed genes (DEGs), with 2278 upregulated and 940 downregulated. Significantly, this study unveiled fluoride’s induction of endoplasmic reticulum (ER) stress in cells, triggering a cascade involving the PERK pathway factor <i>ATF4</i>, leading to cell death via <i>DDIT3</i>/<i>CHOP</i> activation and the subsequent upregulation of <i>CHAC1</i>, <i>ATF3</i>, <i>ERO1α</i>, and <i>TRIB3</i>. These findings provide crucial insights into the toxicity of fluoride in ovine, offering a foundation for mitigating fluoride-related losses in the farming industry.