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Rapeseed isolates of Sclerotinia sclerotiorum (Lib.) de Bary 1884 from Nitra Region of Slovakia were investigated for their in vitro sensitivity to azoxystrobin and picoxystrobin; and determining the EC50 value. The growth of S. sclerotiorum was evaluated on PDA amended with the selected fungicide´s active ingredient at 4 different concentration - 0.08, 0.83, 8.33, and 83.30 ppm. The overall mean EC50 values for azoxystrobin and picoxystrobin were 2.73 ppm and 3.12 ppm respectively. Majority of isolates had a resistance factors up to 20, that suggests the shift in S. sclerotiorum population sensitivity towards the resistance.