Optimized protocol for immunophenotyping of melanoma and tumor-bearing skin from mouse
oleh: Sai Sakktee Krisna, Christophe Goncalves, Natascha Gagnon, Fan Huang, Dany Plourde, Wilson H. Miller, Jr., Jörg H. Fritz, Sonia V. del Rincon
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2021-09-01 |
Deskripsi
Summary: While isolating immune cells from spleens and lungs is routinely achieved using flow cytometry, it is challenging to isolate viable immune cells from skin. Here, we describe a step-by-step protocol for skin digestion using a murine melanoma model, which is amenable for detection of low abundant immune cell populations including group 2 innate lymphoid cells.