In vitro modeling of amyotrophic lateral sclerosis with induced pluripotent stem cell technology-derived cell line ORIONi002-A
oleh: Jan Strnadel, Hugo M. Dumortier, Dominika Hajduchova, Romana Zahumenska, Vladimir Nosal, Marek Smolar, Juraj Marcinek, Michal Kalman, Sandra Mersakova, Dusan Brany, Stefan Juhas, Jana Juhasova, Hana Studenovska, Barbora Mitruskova, Stanislava Suroviakova, Slavomira Novakova, Henrieta Skovierova, Egon Kurca, Renata Pecova, Lukas Plank, Erika Halasova
| Format: | Article |
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| Diterbitkan: | Elsevier 2022-08-01 |
Deskripsi
We present here a new iPS cell line for modeling sporadic form of ALS. Cell line was generated by reprogramming skin fibroblasts isolated with explant culture technology from skin biopsy, donated by ALS patient. For reprogramming, polycistronic self-replicating RNA vector was used and derived iPS cells were characterized by immunocytochemistry and FACS (pluripotent factors expression), karyotyping, STR fingerprinting analysis and in vitro differentiation assay. New cell line showed normal (46, XY) karyotype and differentiated in vitro into cells from three germ layers. STR analysis proved the origin and originality of the cell line.