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The study was carried out to determine the prevalence of bovine sarcocystosis in slaughtered cattle in Babylon abattoir by use two methods, trichenoscopy and histological techniques, The results showed that the prevalence rate of microscopic form Sarcocystis cruzi detected by trichenoscopy and histological technique were  66.5 % and 70% respectively. According to examined organs, the higher percentage recorded in esophagus 64%, and least percentage in diaphragm 47%, also the prevalence in females 79. 03 % was higher than males 60..86%. In addition, the results revealed that the infection rate increased with the age and the higher percentage was observed in animals 2-6 years, while the lowest was recorded in animals aged 6-12 months.