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<strong><strong><em></em></strong></strong><p align="left"><strong>Background</strong>:<span style="font-size: xx-small; font-family: Times New Roman;"><em><span style="font-size: xx-small; font-family: Times New Roman;">Pseudomonas aeruginosa </span></em></span><span style="font-size: xx-small; font-family: Times New Roman;">is a severe challenge for antimicrobial therapy, because </span>of chromosomal mutations or exhibition of intrinsic resistance to various antimicrobial agents such as most β<span style="font-size: xx-small; font-family: Times New Roman;">-lactams. We undertook this study to evaluate the existence of SME, AIM and </span>VIM metallo-beta lactamases encoding genes among <em><span style="font-size: xx-small; font-family: Times New Roman;"><em><span style="font-size: xx-small; font-family: Times New Roman;">P. aeruginosa </span></em></span></em><span style="font-size: xx-small; font-family: Times New Roman;">strains isolated from ICU </span>patients in AL- Zahra Hospital in Esfahan-Iran.</p><p align="left"><strong>Methods</strong> <span style="font-size: xx-small; font-family: Times New Roman;">: In a retrospective cross sectional study that was conducted between March 2012 to</span>April 2013, in total 48 strains of <em><span style="font-size: xx-small; font-family: Times New Roman;"><em><span style="font-size: xx-small; font-family: Times New Roman;">P. aeruginosa </span></em></span></em><span style="font-size: xx-small; font-family: Times New Roman;">were collected from clinical specimens of </span>bedridden patients in ICU wards. Susceptibility test was performed by disc diffusion method.All of the meropenem resistant strains were subjected to modified Hodge test (MHT) for detection of carbapenemases. Multiplex PCR was performed for detection of VIM, blaAIM,blaSME genes.</p><p align="left"><strong>Results <span style="font-size: xx-small; font-family: Times New Roman;"><strong><span style="font-size: xx-small; font-family: Times New Roman;">: </span></strong></span></strong><span style="font-size: xx-small; font-family: Times New Roman;">In disk diffusion method imipenem and meropenem showed the most and colistin the </span>least resistant antimicrobial agents against <em><span style="font-size: xx-small; font-family: Times New Roman;"><em><span style="font-size: xx-small; font-family: Times New Roman;">P. aeruginosa </span></em></span></em><span style="font-size: xx-small; font-family: Times New Roman;">strains. Of the 48 isolates 36, (75%) </span>were multidrug resistant. Amplification of β –<span style="font-size: xx-small; font-family: Times New Roman;">lactamase genes showed the presence of blaVIM </span>genes in 7 (%14.6) strains. All of the isolates were negative for blaSME and blaAIM genes. We ouldn’t find any statistically significance diffe <span style="font-size: xx-small; font-family: Times New Roman;">rence among presence of this gene and MDR </span>positive, age or source of the specimen.</p><p align="left"><strong>Conclusion <span style="font-size: xx-small; font-family: Times New Roman;"><strong><span style="font-size: xx-small; font-family: Times New Roman;">: </span></strong></span></strong><span style="font-size: xx-small; font-family: Times New Roman;">As patients with infections caused by MBL-producing bacteria are at an intensified </span>risk of treatment failure, fast determination of these organisms is necessary. Our findings may rovide useful insights in replace of the appropriate antibiotics and may also prevent MBLs mediated resistance problem.</p><p align="left"> </p>