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An efficient protocol for in vitro regeneration of peanut (Arachis hypogaea L.) cultivar L14
oleh: Phung Thi Bich Hoa, Nguyen Hoang Tue, Phan Thi Quyen Trang, Le Thi Hang, Nguyen Quang Duc Tien, Nguyen Hoang Loc
Format: | Article |
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Diterbitkan: | Universidade Federal de Uberlândia 2021-04-01 |
Deskripsi
The present work aims to establish an efficient protocol for in vitro regeneration of peanut (Arachis hypogaea) cultivar L14. The study showed that de-embryonated cotyledon was a suitable explant for shoot multiplication on MS medium containing 4 mg/L BAP. The highest number of shoots per explant obtained after 4 weeks of culture was up to 6.8 shoots. Shoots in vitro were able to produce a large number of approximately 11 roots on MS medium supplemented with 0.5 mg/L NAA. These results will be very useful in establishing an in vitro regeneration protocol for peanut cultivar L14 during gene transfer in the next studies to improve their disease resistance.