SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation
oleh: Jurre A. Steens, Yifan Zhu, David W. Taylor, Jack P. K. Bravo, Stijn H. P. Prinsen, Cor D. Schoen, Bart J. F. Keijser, Michel Ossendrijver, L. Marije Hofstra, Stan J. J. Brouns, Akeo Shinkai, John van der Oost, Raymond H. J. Staals
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-08-01 |
Deskripsi
Type III CRISPR-Cas systems recognize and cleave target RNAs and produce signalling molecules. Here the authors discover that both processes are governed by a flexible seed region, ultimately resulting in SCOPE, a SARSCoV-2 diagnostic assay with atto-molar sensitivity.