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The geographic distribution of Great Horned Owl, Bubo virginianus (Gmelin, 1788), in Rio de Janeiro state, southeastern Brazil, is revised and expanded. Two individuals were recorded in an Atlantic Forest fragment surrounded by rural and industrial areas in Macaé municipality, on the northern coast of Rio de Janeiro state. This is the twentieth time that B. virginianus is documented in this state in the last two centuries and highlights the importance of bird surveys in regions with ornithological knowledge gaps.