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Rhizoctonia solani, the causal agent of <em>damping</em>-<em>off</em>, <em>root</em> rot and <em>stem rot</em>, is one of the most important phytopathogenes with a wide host range which causes significant reduction in yield of pistachio (<em>Pistacia vera</em>). In search for finding chitinolytic biocontrol agents, actinomycetes producing chitinase enzyme were isolated from agricultural soils of Sirch, Kerman province . From tested isolates, Streptomyces isolate 410 significantly reduced the incidence of disease. Chitinase gene (603 bp) was cloned, sequenced and characterized from this isolate. The results showed that nucleotides encoding 200 amino acids and molecular weight of 21636.9 Da. This protein had the high homology with the family 19 chitinases glycosyl hydrolases which have the major role in the defense response against <em>phytopathogens</em>