Altered ganglioside GD3 in HeLa cells might influence the cytotoxic abilities of NK cells

oleh: Wen-Chi Lee, Wen-Ling Lee, Wen-Yuann Shyong, Lin-Wei Yang, Min-Chun Ko, Chang-Ching Yeh, Shie-Liang Edmond Hsieh, Peng-Hui Wang

Format: Article
Diterbitkan: Elsevier 2012-06-01

Deskripsi

Objective: Previously, we found that altered sialidases in HeLa cells in a natural killer-HeLa (NK-HeLa) coculture system contributed to the decreased cytotoxic ability of NK cells. However, changes that occur in the glycosylation of the HeLa cells in the NK-HeLa coculture system remain unknown. Materials and Methods: An NK-HeLa coculture system was used to examine the changes that occur in the gangliosides of HeLa cells. Results: GD3 expression in HeLa cells was significantly increased in the NK-HeLa coculture system. Exogenous ganglioside GD3 decreased the cytotoxic ability of the NK cells, which could be restored by the addition of the anti-GD3 antibody. Coadministration of GD3 and sialidase further decreased the cytotoxic ability of the NK cells, which could be partially restored by the addition of a sialidase inhibitor (DANA). GD3 expression in HeLa cells also decreased following DANA treatment. Conclusions: This study suggests that interactions between ganglioside GD3 and sialidases in HeLa cells influence the cytotoxic ability of NK cells.