Antioxidant, Antimicrobial, Antidiabetic and Cytotoxic Activity of <i>Crocus sativus</i> L. Petals
oleh: Adil Farooq Wali, Houda Ahmed Abou Alchamat, Huda Khaled Hariri, Bushra Khaled Hariri, Godfred A. Menezes, Uzma Zehra, Muneeb U. Rehman, Parvaiz Ahmad
| Format: | Article |
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| Diterbitkan: | MDPI AG 2020-02-01 |
Deskripsi
The purpose of this research is to examine in vitro antioxidant, antimicrobial, antidiabetic and cytotoxic efficacy of different extracts of <i>Crocus sativus</i> L. petals. Antioxidant activity of extracts was assessed by DPPH and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assay. Among all extracts, ethanol (SPE) had the maximum radical scavenging activity with IC<sub>50</sub> values 86.63 ± 7.53 μg/mL. The antimicrobial activity was determined by the evaluation of the minimum inhibitory concentration using the agar well plate procedure. The most effective extract was SPE with a minimum inhibitory concentration varying between 500 µg/mL, 250 µg/mL, 125 µg/mL, 62.5 µg/mL, 31.25 µg/mL, 15.63 µg/mL. Cytotoxic activity was tested against MDA-MB-231 cell lines using the MTT method whereas, antidiabetic activity was evaluated using an alpha-glucosidase inhibition assay. All extracts were found to have significant antidiabetic activity.