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Lake Lesser Prespa in Greece is part of the Prespa Lakes area which is a Ramsar site and a Special Protection Area due to its international importance for endemic biodiversity and migratory birds. Twenty fish taxa have been reported in that lake. From a field survey in July 2016, the species Economidichthys pygmaeus (Holly, 1929) (Gobiidae) was identified based on a molecular analysis and can be added to the list of fish species inhabiting Lake Lesser Prespa, which is the most northern location where this species has been found in Greece.