Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity
oleh: Duy P. Nguyen, Yuichiro Miyaoka, Luke A. Gilbert, Steven J. Mayerl, Brian H. Lee, Jonathan S. Weissman, Bruce R. Conklin, James A. Wells
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2016-07-01 |
Deskripsi
CRISPR-Cas9 has been widely used to manipulate genomes, however control over activity is necessary to explore transcriptional or epigenetic regulation. Here the authors use a split Cas9 fused to nuclear receptor ligand-binding domains to achieve tuneable Cas9 activity.