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<i>Oxalis triangularis</i> ‘Purpurea’ has significant ornamental value in landscaping. There is a critical necessity to elucidate the gene functions of <i>O. triangularis</i> ‘Purpurea’ and dissect the molecular mechanisms governing key ornamental traits. However, a reliable genetic transformation method remains elusive. In this study, our investigation revealed that various transformation parameters, including recipient material (petioles), pre-culture time (2–5 days), acetosyringone (AS) concentration (100–400 μM), <i>Agrobacterium</i> concentrations (OD₆₀₀ = 0.4–1.0), infection time (5–20 min), and co-culture time (2–5 days), significantly impacted the stable genetic transformation in <i>O. triangular</i> ‘Purpurea’. Notably, the highest genetic transformation rate was achieved from the leaf discs pre-cultured for 3 days, treated with 200 μM AS infected with <i>Agrobacterium</i> for 11 min at OD₆₀₀ of 0.6, and subsequently co-cultured for 3 days. This treatment resulted in a genetic transformation efficiency of 9.88%, and it only took 79 days to produce transgenic plants. Our transformation protocol offers advantages of speed, efficiency, and simplicity, which will greatly facilitate genetic transformation for <i>O. triangular</i> ‘Purpurea’ and gene function studies.