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Background: Revolutionary biological sciences such as biotechnology is taking over the million-dollar industries due to their imminent applications as well as their importance in the well-being of living organisms. However, its scope remains a question for students when they opt for their career after passing out from their respective department. The objective of this study is to determine the factors involved in electing this field, their career preferences, attitude and knowledge regarding biotechnology profession among University students. Methods: The study was conducted in University of Peshawar, Pakistan through a prevalidated questionnaire which was given to the students enrolled in biotechnology department. The sample of 200 students was randomly selected. Their responses were collected, organized in excel sheets and then analyzed through SPSS 17.0. Results: Out of the 200 participants, 168 students filled the questionnaire; therefore, the response rate was 84%. A total of 110 (65.5%) students out of 168 respondents were not aware of the scope of biotechnology before getting admission in biotechnology program. Moderate number of students (62 [36.9%]) paid heed to research, while (16 [9.5%]) were either uninterested or uncertain about their options. Conclusion: Nearly all respondents (74 [44%]) were willing to pursue careers in biotechnology after their graduation and Personal Interest was the notable factor (76%) involved in the selection succeeded by status and income respectively.