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AIM: To study the effect of platelet derived growth factor ɑ receptor(PDGFR-ɑ)antibody on the rust-induced human retinal pigment epithelial cell(hRPE)proliferation. <p>METHODS: <i>In vitro</i> rust hRPE cells proliferation differentiation model was established. Blank control group, rust group, PDGFR-ɑ antibody(1, 10, 50, and 100μg/mL)treatment group were established. The effect of anti-PDGFR-ɑ antibody on the growth of RPE was determined by MTT colorimetric assay after 0, 12, 24, 48h,and the inhibition rates were calculated accordingly. <p>RESULTS: After adding the anti-PDGFR-ɑ antibody, hRPE cells proliferation activity was decreased at certain concentration as the antibody concentration increased, and 50μg/mL anti-PDGFR-ɑ antibody was the optimum concentration to inhibit hRPE cells proliferation, the inhibitory rate of hRPE was 42.44%.<p>CONCLUSION: The anti-PDGFR-ɑ antibody shows strong inhibitory effect on the growth of cultured hRPE.