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Abstract We present the bounds on 3+1 active-sterile neutrino mixing obtained from the first results of the KATRIN experiment. We show that the KATRIN data extend the Mainz and Troitsk bound to smaller values of Δ m 41 2 $$ \Delta {m}_{41}^2 $$ for large mixing and improves the exclusion of the large- Δ m 41 2 $$ \Delta {m}_{41}^2 $$ solution of the Huber-Muller reactor antineutrino anomaly. We also show that the combined bound of the Mainz, Troitsk, and KATRIN tritium experiments and the Bugey-3, NEOS, PROSPECT, and DANSS reactor spectral ratio measurements exclude most of the region in the (sin22ϑ ee , Δ m 41 2 $$ \Delta {m}_{41}^2 $$ ) plane allowed by the Huber-Muller reactor antineutrino anomaly. Considering two new calculations of the reactor antineutrino fluxes, we show that one, that predicts a lower 235U antineutrino flux, is in agreement with the tritium and reactor spectral ratio measurements, whereas the other leads to a larger tension than the Huber-Muller prediction. We also show that the combined reactor spectral ratio and tritium measurements disfavor the Neutrino-4 indication of large active-sterile mixing. We finally discuss the constraints on the gallium neutrino anomaly.