Find in Library
Search millions of books, articles, and more
Indexed Open Access Databases
Calcium Dynamics in Astrocytes During Cell Injury
oleh: Nicole M. Wakida, Veronica Gomez-Godinez, Huayan Li, Jessica Nguyen, Edward K. Kim, Joseph L. Dynes, Shivashankar Othy, Alice L. Lau, Peng Ding, Linda Shi, Christopher Carmona, Leslie M. Thompson, Leslie M. Thompson, Leslie M. Thompson, Leslie M. Thompson, Michael D. Cahalan, Michael D. Cahalan, Michael W. Berns, Michael W. Berns, Michael W. Berns
Format: | Article |
---|---|
Diterbitkan: | Frontiers Media S.A. 2020-08-01 |
Deskripsi
The changes in intracellular calcium concentration ([Ca2+]) following laser-induced cell injury in nearby cells were studied in primary mouse astrocytes selectively expressing the Ca2+ sensitive GFAP-Cre Salsa6f fluorescent tandem protein, in an Ast1 astrocyte cell line, and in primary mouse astrocytes loaded with Fluo4. Astrocytes in these three systems exhibit distinct changes in [Ca2+] following induced death of nearby cells. Changes in [Ca2+] appear to result from release of Ca2+ from intracellular organelles, as opposed to influx from the external medium. Salsa6f expressing astrocytes displayed dynamic Ca2+ changes throughout the phagocytic response, including lamellae protrusion, cytosolic signaling during vesicle formation, vesicle maturation, and vesicle tract formation. Our results demonstrate local changes in [Ca2+] are involved in the process of phagocytosis in astrocytes responding to cell corpses and/or debris.