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<i>Erwinia amylovora</i> is a Gram-negative bacterium, responsible for the fire blight disease in Rosaceae plants. Its virulence is correlated with the production of an exopolysaccharide (EPS) called amylovoran, which protects the bacterium from the surrounding environment and helps its diffusion inside the host. Amylovoran biosynthesis relies on the expression of twelve genes clustered in the <i>ams</i> operon. One of these genes, <i>amsI</i>, encodes for a Low Molecular Weight Protein Tyrosine Phosphatase (LMW-PTP) called <i>Ea</i>AmsI, which plays a key role in the regulation of the EPS production pathway. For this reason, <i>Ea</i>AmsI was chosen in this work as a target for the development of new antibacterial agents against <i>E. amylovora</i>. To achieve this aim, a set of programs (DOCK6, OpenEye FRED) was selected to perform a virtual screening using a database of ca. 700 molecules. The six best-scoring compounds identified were tested in in vitro assays. A complete inhibition kinetic characterization carried out on the most promising molecule (n-Heptyl β-D-glucopyranoside, N7G) showed an inhibition constant of 7.8 ± 0.6 µM. This study represents an initial step towards the development of new <i>Ea</i>AmsI inhibitors able to act as antibacterial agents against <i>E. amylovora</i> infections.