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Recently, there has been an increasing interest among researchers in using combinations of biological control agents to exploit potential synergistic effects among them. In the present study, there were investigated commercially a</span><span lang=EN-US style='font-size:10.0pt;font-family:"Garamond Premr Pro","serif"; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Garamond Premr Pro Subh"; color:black;mso-ansi-language:EN-US;mso-fareast-language:EN-US;mso-bidi-language: AR-SA'>cceptable formulations of <i style='mso-bidi-font-style:normal'><span style='mso-bidi-font-weight:bold'>Bacillus cereus</span></i> CE3 wetting powder with long storage life and retained efficacy to control chestnut and other fruit rot caused by <i>Endothia parasitica</i><span style='mso-bidi-font-style:italic'> </span>(Murr) and <i>Fusarium solani</i><span style='mso-bidi-font-style:italic'>.</span><b style='mso-bidi-font-weight: normal'> </b><span style='mso-bidi-font-weight:bold'>The study sought to develop a new <i style='mso-bidi-font-style:normal'>B. cereus</i> formulation that would be more effective and better suited to the conditions of field application. By a series of experiments,<span style='mso-bidi-font-style:italic'> </span></span>the formulation was confirmed as follows: 60% <i>B. cereus</i> freeze-dried powder, 28.9% diatomite as carrier, 4% sodium lignin sulfonate as disperser, 6% <span style='mso-bidi-font-weight:bold'>alkyl naphthalene sulfonate</span> as wetting agent, 1% K₂HPO₄as stabilizer, 0.1% </span><span lang=EN-US style='font-size:10.0pt;font-family: "Garamond Premr Pro","serif";mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US; mso-fareast-language:EN-US;mso-bidi-language:AR-SA'>&#946;</span><span lang=EN-US style='font-size:10.0pt;font-family:"Garamond Premr Pro","serif";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:"Garamond Premr Pro";color:black; mso-ansi-language:EN-US;mso-fareast-language:EN-US;mso-bidi-language:AR-SA'>-cyclodextrin as ultraviolet protectant. The controlling experiments showed that the diluted 100 times of 60% </span><i><span lang=EN-US style='font-size:10.0pt;font-family: "Garamond Premr Pro","serif";mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Garamond Premr Pro Subh";color:black;mso-ansi-language: EN-US;mso-fareast-language:EN-US;mso-bidi-language:AR-SA'>B. cereus</span></i><span lang=EN-US style='font-size:10.0pt;font-family:"Garamond Premr Pro","serif"; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Garamond Premr Pro Subh"; color:black;mso-ansi-language:EN-US;mso-fareast-language:EN-US;mso-bidi-language: AR-SA'> <span style='mso-bidi-font-weight:bold'>wetting powder </span>had 79.47% corrosion rate to <span style='mso-bidi-font-weight:bold'>chestnut pathogens; and this result is comparable to the diluted 1,000 times of 70% thiophanate-methyl. Safety evaluation results showed that </span>rats acute oral <span style='mso-bidi-font-weight:bold'>lethal dose 50% was 5,000.35, therefore application of <i style='mso-bidi-font-style:normal'>B. cereus</i> wettable powder could not cause a person or animal poisoning. This work illustrated that 60% <i style='mso-bidi-font-style:normal'>B. cereus</i> wetting powder had commercial potential; however, to apply this formulation as a biological pesticide in the field, masses production processes need to be further studied.</span></span>