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This study evaluates the in vitro probiotic characteristics of <i>P. konkukensis</i> sp. nov. SK-3146, which was isolated from animal feed, and its dietary effects on growth performance, intestinal characteristics, intestinal microbiota, and meat quality in broilers. In vitro experiments revealed that <i>P. konkukensis</i> was non-hemolytic with variable antibiotic susceptibility, and acid as well as bile tolerance. To assess the effect of <i>P. konkukensis</i> on broilers, a total of four hundred eighty 1-day-old Ross 308 broiler chicks were allocated to 3 treatment groups with 4 replicates of 40 birds each; the negative control group was fed a basal diet without any feed additives (NC), the positive control group was fed a basal diet containing 0.01% enramycin (PC), and the experimental group was fed a basal diet containing <i>P. konkukensis</i> bacterial culture (PK) at 10⁴ CFU/g of the diet based on bacterial count. The experiment lasted for 35 days. Results indicated that there were no significant differences in any growth performance parameters among the dietary treatments (<i>p</i> > 0.05). In addition, the inclusion of <i>P. konkukensis</i> in the broilers’ diet did not affect meat cooking loss, color, and pH but increased the relative weight of breast meat (<i>p</i> < 0.05). The PK group showed heavier intestinal weight and shorter intestinal length than the NC group (<i>p</i> < 0.05). The ratio of the intestinal weight to length of jejunum was the highest in the PK group (<i>p</i> < 0.05). The PK group showed increased counts of <i>Streptococcus thermophilus</i> (<i>p</i> < 0.05) with no adverse effects of <i>P. konkukensis</i> on other intestinal microbiota in the jejunum. This study implies that <i>P. konkukensis</i> might have the potential to be applied as a probiotic feed additive in poultry.