Generation of a DKK1 homozygous knockout human embryonic stem cell line WAe001-A-21 using CRISPR/Cas9
oleh: Zhen Yang, Fang Yuan, Ning Wang, Yanli Liu, Jiaye Zhang, Ruzhi Wei, FeiMa Wu, Yuhang Wu, Kai You, Yan Chen, Fan Yang, Yin-xiong Li
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2021-03-01 |
Deskripsi
Dickkopf1 (DKK1) is a secreted inhibitor for the Wnt signalling, which is involved in cell proliferation, tissue regeneration and embryonic development. Using CRISPR/Cas9 editing, we established a homozygous mutant DKK1 human embryonic stem cell line (WAe001-A-21). It has a 41 bp deletion in exon 2 of DKK1, leading to its coding frame shift. The WAe001-A-21 cell line maintains a normal karyotype, pluripotency markers, typical stem cell morphology and the ability to differentiate into three germ layers.