Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex
oleh: Arash Ahmadi, Katharina Till, Paul Hoff Backe, Pernille Blicher, Robin Diekmann, Mark Schüttpelz, Kyrre Glette, Jim Tørresen, Magnar Bjørås, Alexander D. Rowe, Bjørn Dalhus
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-07-01 |
Deskripsi
Ahmadi et al. use a single-molecule tracking method to describe the DNA scanning mode of AlkD, a HEAT-like repeat DNA glycosylase. They show that, contrary to other glycosylases that use a base-flipping mechanism, AlkD scans the DNA without forming a stable interrogation complex.