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A High-Throughput NanoBiT-Based Serological Assay Detects SARS-CoV-2 Seroconversion
oleh: Taha Azad, Reza Rezaei, Ragunath Singaravelu, Taylor R. Jamieson, Mathieu J. F. Crupi, Abera Surendran, Joanna Poutou, Parisa Taklifi, Juthaporn Cowan, Donald William Cameron, Carolina S. Ilkow
Format: | Article |
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Diterbitkan: | MDPI AG 2021-03-01 |
Deskripsi
High-throughput detection strategies for antibodies against SARS-CoV-2 in patients recovering from COVID-19, or in vaccinated individuals, are urgently required during this ongoing pandemic. Serological assays are the most widely used method to measure antibody responses in patients. However, most of the current methods lack the speed, stability, sensitivity, and specificity to be selected as a test for worldwide serosurveys. Here, we demonstrate a novel NanoBiT-based serological assay for fast and sensitive detection of SARS-CoV-2 RBD-specific antibodies in sera of COVID-19 patients. This assay can be done in high-throughput manner at 384 samples per hour and only requires a minimum of 5 μL of serum or 10 ng of antibody. The stability of our NanoBiT reporter in various temperatures (4–42 °C) and pH (4–12) settings suggests the assay will be able to withstand imperfect shipping and handling conditions for worldwide seroepidemiologic surveillance in the post-vaccination period of the pandemic. Our newly developed rapid assay is highly accessible and may facilitate a more cost-effective solution for seroconversion screening as vaccination efforts progress.