HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system.
oleh: Woottichai Khamduang, Ampika Kaewbundit, Amonrat Duangmano, Sayamon Hongjaisee, Virat Klinbuayaem, Guttiga Halue, Apichat Chutanunta, Wasna Sirirungsi, Gonzague Jourdain, Nicole Ngo-Giang-Huong
| Format: | Article |
|---|---|
| Diterbitkan: | Public Library of Science (PLoS) 2020-01-01 |
Deskripsi
World Health Organization recommends using dried blood spots (DBS) for HIV RNA viral load (VL) measurement whenever plasma processing is not convenient or feasible. DBS collected from 80 treatment-naïve HIV-infected patients presenting in three hospitals of two different regions of Thailand were shipped to a central laboratory along with corresponding plasma specimens. Viral load was measured in both DBS and plasma using the Abbott m2000 system. HIV RNA levels were strongly correlated (r = 0.94) with a mean of differences of 0.23 log10 copies/mL. Using the 1,000 copies/mL cut-off, the sensitivity of DBS was 97% (95%CI, 91-100%) and specificity was 75% (95%CI, 19-99%). DBS are useful to scale-up HIV RNA VL testing in settings with limited access to VL testing.