A single-molecule view of transcription reveals convoys of RNA polymerases and multi-scale bursting
oleh: Katjana Tantale, Florian Mueller, Alja Kozulic-Pirher, Annick Lesne, Jean-Marc Victor, Marie-Cécile Robert, Serena Capozi, Racha Chouaib, Volker Bäcker, Julio Mateos-Langerak, Xavier Darzacq, Christophe Zimmer, Eugenia Basyuk, Edouard Bertrand
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2016-07-01 |
Deskripsi
HIV-1 viral gene expression stochastically switches between active and inactive states. Here, using improved single molecule RNA microscopy, the authors show that HIV-1 RNA stochastic transcription is achieved by groups of closely spaced polymerases, and is regulated by Mediator and TBP at different time scales.