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(SMV) is a prevalent virus infecting soybean ( L. Merr) worldwide. The incorporation of , conferring resistance to all currently known strains in the United States, can assist in creating durable virus resistance in soybean. Additionally, lines heterozygous at the locus often express a late susceptible phenotype, showing symptoms only in mid to late vegetative growth. In this study the whole-genome shotgun sequence (WGS) of soybean was utilized for fine mapping and examining potential gene candidates in two populations. Six markers, designed from the WGS, were used to localize in the same, 1.3-cM region in both mapping populations, a physical interval of less than 100 kb on chromosome 2. This region contained no sequences previously related to virus resistance, namely nucleotide binding site-leucine rich repeat gene sequences or eukaryotic translation initiation factors. Instead, sequence analysis revealed several predicted transcription factors and unknown protein products. We conclude that likely belongs to a new class of resistance genes that interfere with viral infection and cell-to-cell movement, and delay vascular movement.