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Whole Genome Sequencing and Pan-Genomic Analysis of Multidrug-Resistant <i>Vibrio cholerae</i> VC01 Isolated from a Clinical Sample
oleh: Vishal Mevada, Rajesh Patel, Pravin Dudhagara, Rajesh Chaudhari, Mustafa Vohra, Vikram Khan, Douglas J. H. Shyu, Yih-Yuan Chen, Dolatsinh Zala
Format: | Article |
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Diterbitkan: | MDPI AG 2023-08-01 |
Deskripsi
Cholera, a disease caused by the <i>Vibrio cholerae</i> bacteria, threatens public health worldwide. The organism mentioned above has a significant historical record of being identified as a prominent aquatic environmental pollutant capable of adapting its phenotypic and genotypic traits to react to host patients effectively. This study aims to elucidate the heterogeneity of the sporadic clinical strain of <i>V. cholerae</i> VC01 among patients residing in Silvasa. The study involved conducting whole-genome sequencing of the isolate obtained from patients exhibiting symptoms, including those not commonly observed in clinical practice. The strain was initially identified through a combination of biochemical analysis, microscopy, and 16s rRNA-based identification, followed by type strain-based identification. The investigation demonstrated the existence of various genetic alterations and resistance profiles against multiple drugs, particularly chloramphenicol (<i>catB9</i>), florfenicol (<i>floR</i>), oxytetracycline (<i>tet(34)</i>), sulfonamide (<i>sul2</i>), and Trimethoprim (<i>dfrA1</i>). The pan-genomic analysis indicated that 1099 distinct clusters were detected within the genome sequences of recent isolates worldwide. The present study helps to establish a correlation between the mutation and the coexistence of antimicrobial resistance toward current treatment.