Phenylalanine biosynthesis and its relationship to accumulation of capsaicinoids during Capsicum chinense fruit development
oleh: L. A. Castro-Concha, F. M. Baas-Espinola, W. R. Ancona-Escalante, F. A. Vázquez-Flota, M. L. Miranda-Ham
| Format: | Article |
|---|---|
| Diterbitkan: | Institute of Experimental Botany of the Czech Academy of Sciences 2016-09-01 |
Deskripsi
Activities of phenylalanine (Phe) biosynthetic enzymes chorismate mutase (CM) and arogenate dehydratase (ADT) and of phenylalanine ammonia lyase [PAL, an enzyme that directs Phe towards capsaicinoid (CAP) synthesis] were analyzed during Capsicum chinense Jacq. (habanero pepper) fruit development. A maximum CM activity coincided with a maximum CAP accumulation. However, ADT exhibited two activity peaks, one during the early phase (10 - 17 days post-anthesis, DPA) and another during the late phase (35 - 37 DPA); only the latter coincided with CAP. Interestingly, PAL activity was inversely related to CAP accumulation; lower activities coincided with a maximum CAP content. These results suggest the operation of a control mechanism that coordinated Phe synthesis and its channeling towards CAP synthesis during the course of fruit development.