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Focusing on the Yangtze River Delta region, the spatial distribution and change characteristics of the employed population were assessed by selecting three time points: 2000, 2010 and 2020. Firstly, a correlation was established between population employment statistics and spatial units of administrative divisions to analyze the spatial distribution characteristics of the employed population in general and by industry; secondly, the changing characteristics of the spatial distribution of the employed population over time, including the migration of the centroid and density changes, were analyzed; thirdly, a systematic clustering approach was adopted to carry out a typological analysis of 41 cities in the Yangtze River Delta from three perspectives: industrial structure, time stage and spatial level. It was found that (1) regional differences within the Yangtze River Delta are still significant, but are narrowing; (2) different cities or regions show different characteristics of development stages, and late-developing regions can learn from early developing regions; (3) metropolitan areas are still the main areas of employment concentration, and the spatial distribution of employment in some cities is beginning to suburbanize.