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The aim of this research was to establish the influence of breed, parity and food intake on chemical composition of first colostrum. We observed that fat, proteins, lactose and dry matter were higher in cows from second and third lactation compared to those in fourth lactation. Cow's breed also influenced the colostrum composition, superior quality being obtained in case of Romanian White and Black comparing Holstein Friesian cows. The unbalanced energo-proteic ratio had a negative impact on chemical composition of first colostrum.