A growth selection system for the directed evolution of amine-forming or converting enzymes
oleh: Shuke Wu, Chao Xiang, Yi Zhou, Mohammad Saiful Hasan Khan, Weidong Liu, Christian G. Feiler, Ren Wei, Gert Weber, Matthias Höhne, Uwe T. Bornscheuer
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-12-01 |
Deskripsi
Fast screening of enzymes is key for directed evolution of industrial biocatalysts. Here, the authors report a simple, high-throughput, and low-equipment-dependent growth selection system for engineering three enzymes for synthesis of chiral amines.