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Intravitreal injection is one of the most commonly used treatment methods in ophthalmology at present. It is easy to operate and has a shorter operation time. However, there is no consensus on whether it is necessary to dilate pupils before intravitreal injection. For this reason, we conducted an online questionnaire survey. On the basis of relevant research, we discussed the above issues and put forward our proposals. It is not necessary to dilate pupils before intravitreal injection.