Polymorphisms of the <i>HRG</i>, <i>FETUB</i>, and <i>GUCY1A1</i> genes and their association with litter size in sheep
oleh: Z. Ren, X. He, X. Wang, M. Chu
| Format: | Article |
|---|---|
| Diterbitkan: | Copernicus Publications 2024-04-01 |
Deskripsi
<p>Litter size is one of the key factors affecting the efficiency of sheep breeding, and previous studies found that the <i>HRG</i>, <i>FETUB</i>, and <i>GUCY1A1</i> genes were closely related to litter size in sheep. This experiment aims to explore the polymorphisms of the g.405442728A<span class="inline-formula"><i>></i></span>G locus of the <i>HRG</i> gene, the g.421655951C<span class="inline-formula"><i>></i></span>T locus of the <i>FETUB</i> gene, and the g.414050897G<span class="inline-formula"><i>></i></span>C locus of the <i>GUCY1A1</i> gene and their association with sheep litter size. The MassARRAY<sup>®</sup> single-nucleotide polymorphism (SNP) genotyping technique was used to detect the polymorphisms of these loci in five sheep breeds, i.e., Small-tailed Han sheep, Hu sheep, Cele black sheep, Sunite sheep, and Bamei mutton sheep. In addition, the association between the polymorphisms of these genes and the litter size of Small-tailed Han sheep was also analyzed. The results showed that the g.405442728A<span class="inline-formula"><i>></i></span>G locus of the <i>HRG</i> gene was moderately polymorphic (0.25 <span class="inline-formula"><i><</i></span> PIC <span class="inline-formula"><i><</i>0.5</span>) in both monotocous and polytocous sheep breeds; the g.421655951C<span class="inline-formula"><i>></i></span>T locus of the <i>FETUB</i> gene was lowly polymorphic (PIC <span class="inline-formula"><i><</i>0.25</span>) in five sheep breeds; the g.414050897G<span class="inline-formula"><i>></i></span>C locus of <i>GUCY1A1</i> showed moderately polymorphism in Small-tailed Han sheep (0.25 <span class="inline-formula">≤</span> PIC <span class="inline-formula"><i><</i>0.5</span>) and low polymorphism in four other sheep breeds (PIC <span class="inline-formula"><i><</i>0.25</span>). The chi-squared test results showed that the g.405442728A<span class="inline-formula"><i>></i></span>G locus of the <i>HRG</i> gene was in the Hardy–Weinberg equilibrium state in five sheep breeds (<span class="inline-formula"><i>P</i><i>></i>0.05</span>). The g.421655951 C<span class="inline-formula"><i>></i></span>T locus of the <i>FETUB</i> gene and the g.414050897G<span class="inline-formula"><i>></i></span>C locus of the <i>GUCY1A1</i> gene were in the Hardy–Weinberg equilibrium state in Small-tailed Han sheep (<span class="inline-formula"><i>P</i><i>></i>0.05</span>) and in the Hardy–Weinberg disequilibrium state in other sheep breeds (<span class="inline-formula"><i>P</i><i><</i>0.05</span>). The association analysis showed that the g.405442728A<span class="inline-formula"><i>></i></span>G locus of the <i>HRG</i> gene and the g.421655951C<span class="inline-formula"><i>></i></span>T locus of the <i>FETUB</i> gene had a significant impact on the litter size of sheep (<span class="inline-formula"><i>P</i><i><</i>0.05</span>), while the g.414050897G<span class="inline-formula"><i>></i></span>C locus of the <i>GUCY1A1</i> gene had no significant impact on the litter size (<span class="inline-formula"><i>P</i><i>></i>0.05</span>). In summary, the <i>HRG</i> gene and the <i>FETUB </i>gene can be used as potential molecular markers for the selection of the litter size in sheep.</p>