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Global fitting for high-accuracy multi-channel single-molecule localization
oleh: Yiming Li, Wei Shi, Sheng Liu, Ivana Cavka, Yu-Le Wu, Ulf Matti, Decheng Wu, Simone Koehler, Jonas Ries
Format: | Article |
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Diterbitkan: | Nature Portfolio 2022-06-01 |
Deskripsi
Multi-channel SMLM imaging is powerful. Here the authors report globLoc, a GPU-based global fitting algorithm, to extract maximum information from multichannel single molecule data; this gives improved localisation precision for biplane and 4Pi-SMLM and colour assignment in multi-colour astigmatic SMLM.