Global fitting for high-accuracy multi-channel single-molecule localization

oleh: Yiming Li, Wei Shi, Sheng Liu, Ivana Cavka, Yu-Le Wu, Ulf Matti, Decheng Wu, Simone Koehler, Jonas Ries

Format: Article
Diterbitkan: Nature Portfolio 2022-06-01

Deskripsi

Multi-channel SMLM imaging is powerful. Here the authors report globLoc, a GPU-based global fitting algorithm, to extract maximum information from multichannel single molecule data; this gives improved localisation precision for biplane and 4Pi-SMLM and colour assignment in multi-colour astigmatic SMLM.