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<i>Arthrinium phaeospermum</i> is the major pathogen responsible for the significant stem disease “blight” in <i>B. pervariabilis</i> × <i>D. grandis</i>. The interacting proteins of the key pathogenic factor <i>ApCtf1β</i>, BDUbc and BDSKL1, have previously been obtained by two-hybrid, BiFC, GST pull-down yeast assays. However, the functions of these interacting proteins remain unknown. This study successfully obtained transgenic plants overexpressing <i>BDUbc</i>, <i>BDSKL1</i>, and <i>BDUbc</i> + <i>BDSKL1</i> via Agrobacterium-mediated gene overexpression. qRT-PCR analysis revealed significantly increased expression levels of <i>BDUbc</i> and <i>BDSKL1</i> in the transgenic plants. After infection with the pathogenic spore suspension, the disease incidence and severity index significantly decreased across all three transgenic plants, accompanied by a marked increase in defense enzyme levels. Notably, the co-transformed plant, OE-<i>BDUbc</i> + <i>BDSKL1</i>, demonstrated the lowest disease incidence and severity index among the transgenic variants. These results not only indicate that <i>BDUbc</i> and <i>BDSKL1</i> are disease-resistant genes, but also that these two genes may exhibit a synergistic enhancement effect, which further improves the resistance to blight in <i>Bambusa pervariabilis</i> × <i>Dendrocalamopsis grandis</i>.