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As one of the key step of spacecraft propulsion system assembly, globe-cone joint assembly determines the spacecraft’s high reliability and long life on orbit. Through test, simulation analysis and calculation on sealing mechanism of globe-cone joint, in this paper, we make sure the relationship of the tightening torque and globe-cone joint contact stress and contact area, and obtain assembly torque range of different specifications of globe-cone joint, which gives necessary reference for mechanical environment verification.