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Subcritical Water Enhanced with Deep Eutectic Solvent for Extracting Polysaccharides from <i>Lentinus edodes</i> and Their Antioxidant Activities
oleh: Jixian Zhang, Zhiqiang Ye, Guoyan Liu, Li Liang, Chaoting Wen, Xiaofang Liu, Youdong Li, Tao Ji, Dongming Liu, Jiaoyan Ren, Xin Xu
Format: | Article |
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Diterbitkan: | MDPI AG 2022-06-01 |
Deskripsi
In the present study, subcritical water extraction (SWE) assisted with deep eutectic solvent (DES) is used to extract <i>Lentinus edodes</i> polysaccharides (LEP). In addition, the antioxidant activity of the polysaccharide samples was also investigated. Based on a single factor test and response surface test, the optimal extraction factors were a liquid–solid solvent of 40:1 mL/g, extraction temperature of 147.23 °C, water content of 39.76% and extraction time of 17.58 min. Under these extraction conditions, the yield of LEP was 6.26 ± 0.08%. Compared with the SWE and hot water extraction (HWE), it improved by 19.24% and 17.01%, respectively. In addition, the results of monosaccharide composition, molecular weight, FT-IR, UV and SEM confirmed that the extracts had the features of polysaccharides. Interestingly, the polysaccharides obtained with the SWE assisted with the DES procedure showed a higher DPPH scavenging activity, hydroxyl radical scavenging activity and hydrogen peroxide scavenging activity, which indicated that the polysaccharides with this method had a stronger antioxidant activity. These findings demonstrated that the SWE-assisted DES is a strong method to obtain polysaccharides from <i>Lentinus edodes</i> for food, biopharmaceutical and other industrial production.