Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition
oleh: Zhong Yao, Farzaneh Aboualizadeh, Jason Kroll, Indira Akula, Jamie Snider, Anna Lyakisheva, Priscilla Tang, Max Kotlyar, Igor Jurisica, Mike Boxem, Igor Stagljar
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-05-01 |
Deskripsi
Protein-protein interactions are fundamental to the regulation of protein activity and cellular phyisology. Here the authors present Split Intein-Mediated Protein Ligation, which uses bait and prey proteins fused to intein fragments to generate single intact proteins upon interaction.