CRISPR-induced DNA reorganization for multiplexed nucleic acid detection
oleh: Margot Karlikow, Evan Amalfitano, Xiaolong Yang, Jennifer Doucet, Abigail Chapman, Peivand Sadat Mousavi, Paige Homme, Polina Sutyrina, Winston Chan, Sofia Lemak, Alexander F. Yakunin, Adam G. Dolezal, Shana Kelley, Leonard J. Foster, Brock A. Harpur, Keith Pardee
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2023-03-01 |
Deskripsi
Nucleic acid sensing involving CRISPR technologies is powerful but has certain limitations, such as PAM sequence requirements and limited multiplexing. Here, authors report a CRISPR-based barcoding technology which enables multiple outputs from any target sequence, based on cis- and trans-cleavage.